Closed femur fractures were produced in the right hind limb of mice using an established procedure (Manigrasso and O'Connor 2004).
We used an established procedure to augment cytosolic cAMP level by treating S2 cells with a combination of forskolin, an activator of membrane adenylyl cyclase, and IBMX, an inhibitor of PDEs [ 11].
Admittedly it will not change the order of magnitude of the results, but the problematic gluing of the gap between 1 ns and 20 ns by extrapolating a non-finished double exponential (fit parameters?) can be avoided by determining the effective CO dissociation yield at ~20 ns in the linear excitation regime using an established procedure (Brunori et al., 1973 ).
Endovascular thrombectomy is an established procedure used to treat ischemic stroke.
The quantitative real time PCR was performed according to an established procedure using the TaqMan system [ 34] (PCR, ABI 7700, PE Applied Biosystems, Rotkreuz, Switzerland).
Development of the PreMaPEQ followed an established procedure used previously by NOKC for developing data-collection tools and routines for conducting surveys in new target groups.
The method of data processing, rejection, and analysis of the optical data follows an established procedure used in previous research (Blasi et al. 2007; Lloyd-Fox et al. 2009, 2011).
To assess whether we could model the evolution of the reduced Buchnera network with this key interdependence, we adopted an established procedure using E. coli iJR904 as an approximation to the ancestral non-symbiotic metabolic network [ 19] and applied a sequential gene deletion method to evolve organisms with the same metabolic outputs as iGT196.
This is the first report of a fully diastereoselective reduction of each of the diastereomeric 2-fluorocyclohexanones. 18c, 21 The relative H-bond acidities p KAHY of 1– 10 (Table 1, Figure 1) were determined by adapting an established procedure using FTIR spectroscopy.
This use of authors from different disciplinary backgrounds is an established procedure for improving validity in qualitative studies.
