The first-strand cDNA amplification in both non-infected and infected wasps was performed by using an AMP-like gene-specific primer and the universal primer 3AP according to the method described [ 119].
He is critical of performers who do not connect with their fans, particularly those who "[hide] behind the amps" and use an autocue, remarking that "people pay good money and [they] can't even remember the sodding words".
While it doesn't use AMP as a ranking signal today, it does prominently feature AMP pages.
The RNA was then labeled with Cy3 or Cy5 using an Agilent Quick Amp labeling kit (Product #5190-0444).
Total RNAs were amplified and labeled by using an Agilent Quick Amp labeling kit (part number 5190 0444, Santa Clara, CA) and following the two color protocol (v.5.7).
Heart rate was measured by the R-R wave interval from ECG Lead II using an animal bio amp (FE136; AD Instruments) with needle electrodes inserted into fore and hind limbs (MLA1213; AD Instruments).
This results indicate that the proposed algorithm in this project is suitable to be used as an AMPs predictor.
Also, while playing live, he used an MXR Micro-Amp, Electro-Harmonix Big Muff Pi distortion/sustainer, and an Electro-Harmonix POG (a polyphonic octave generator).
Genomic DNA was isolated from cell aliquots using an Extract-N-Amp Tissue PCR kit (Sigma-Aldrich).
The genotypes of rats were determined by polymerase chain reaction (PCR) of genomic DNA extracted from tail tissue using an REDExtract-N-Amp Tissue PCR kit (R4775; Sigma, St . Louis MO, USA).
