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More recently, heterologous prime-boost regimens mainly use a viral vector or a DNA vaccine for priming, followed by a boost with a protein-based vaccine.
Even though we did not use a viral vector, the presence of the strong viral enhancer/promoter is still a potential concern.
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Two different shRNAs against Sec61β were individually introduced into COS-7 cells using a viral vector.
Thus, an effluc reporter gene was additionally adopted for tracking the migration of DCs and dual reporter DCs expressing both the NIS and effluc genes were successfully established using a viral vector system expressing the reporter genes.
We observe that intrastriatal delivery of NGF and BDNF using a viral vector system can mitigate, albeit only moderately, neuronal death following stroke, which leads to detectable functional sparing.
Although it's possible to deliver CRISPR's components into cells in a dish as proteins or RNA, so far researchers can usually only get it working in tissue inside the body by using a viral vector to deliver the DNA for Cas9 into cells.
The three teams, headed by Yamanaka, the Massachusetts Institute of Technology's Rudolf Jaenisch, and Harvard's Konrad Hochedlinger, all began by following Yamanaka's procedure, using a viral vector to introduce copies of genes for four transcription factors active in ES cells: Oct4, Sox2, c-Myc, and Klf4.
They can be transformed into organisms in the form of plasmids or in the appropriate format, by using a viral vector.
Small hairpin RNA was used to specifically silence the endogenous PRPF3 protein while simultaneously expressing HA-tagged human wild-type or Thr494Met PRPF3, using a viral vector HD-Ad-F3iplus or HD-Ad-F3iT494M HD-Ad-F3iT494M HD-Ad-F3iT494M
This system uses a viral vector, RCAS, derived from the avian leukosis virus (ALV) [76].
This problem can be overcome using a viral vector with mechanisms for nuclear internalisation in non-dividing cells [ 46].
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