He didn't want to use a very stringent design process, he wanted the unexpected phenomenon to occur – and use that".
Third, while Takahashi et al. do not use an analysis strategy that calculates a probability of differential expression, they do use a very stringent sample-wise voting strategy (requiring greater than three-fold differential expression in more than 75% of the patient to normal comparisons) that may in fact be more stringent than the parametric statistical methods we used.
However, using a very stringent filter approach, we found that 44 out of the 177 pre-miRNAs having no SNPs including osa-miR156e-j, osa-miR160a, osa-miR166b, f, osa-miR172a, c, d, osa-miR395j, l, and etc. are highly conserved in plants.
By gene expression analysis using a very stringent threshold, we identified 26 genes, all significantly downregulated, after SOX11 knockdown in MCL cells.
Using a very stringent B value threshold (a Bayesian approach to calculate log-odds of a gene being truly differentially expressed, [30], [31], we found 150 different ESTs being differentially expressed with a very high level of significance (Table S1).
However, a significant number of additional SNPs (60,253) were identified using a very stringent threshold for the quality of the nucleotide used to call the SNP but where the minor allele was only seen twice.
Models were fitted using all of the data ('full' models), and also using a very stringent jack-knife approach in which the data from all of the grid squares of each in turn of the 6° latitudee×8° latitude 'panels' of the UTM grid system were excluded and the model fitted to the remaining data was then used to predict the species' occurrence in the omitted cells.
Their array had limited coverage and they used a very stringent threshold to define differentially expressed genes.
One hundred and seventy-six associations between miRNAs and cellular conditions were identified when using a very stringent threshold (Fig. 4).
Even though we used a very stringent definition of erosion, the prevalence of erosions may thus be overestimated.
To focus on the most significant DMRs, we used a very stringent P-value threshold of 10-18, corresponding to a false discovery rate ≤ 10-15 (Additional file 7).
