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Use a UV filter or a lens hood to protect your lens from sand or loose debris.
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Hoechst stained nuclei were visualized using a UV filter cube DAPI-1160AA, SemRochesterhester, NY) while GFP-MSH6 was visualized using a GFP filter cube (Filter Set 13, Carl Zeiss MicroImaging, Thornwood, NY).
Propidium iodide was detected in protoplasts using a UV filter cube.
For measurement of cell plate angle and apical cell length, plants regenerated from protoplasts were stained with 0.1 mg ml−1 calcofluor solution and visualized by epifluorescence microscopy (Leica MZ16FA, Leica Microsystems, Buffalo Grove, IL) using a UV filter (excitation 360/40 nm, emission 420 long pass) or a Violet filter (excitation 425/40 nm, emission 400 long pass).
FluoroGold-labeled RGCs were counted under an Axioplan microscope (Zeiss) using a UV filter (365/420 nm) per visual field in the central region of the retina (0 1 mm from the optic nerve head along the retina diameter; six visual fields per retina).
For a DSLR, consider using a UV filter.
Nuclear chromatin was visualized using a UV-2E/C filter (325-375 nm) [ 50].
Mice carrying transposon insertions, which activated the polyA trap, were noninvasively identified by visualizing GFP expression in newborn mice under UV light with confirmation by the presence of GFP fluorescence in ear biopsies using a fluorescent stereomicroscope equipped with a UV filter.
Measurements were carried out using an inverse Olympus microscope (IX81) with a UV filter connected to a monochromator (Polychrome II, TILL Photonics).
PI solution was added (2 5 μg/mL) just before observation using an Olympus microscope equipped with epifluorescence and a UV filter block.
Both attached and detached cells were stained and examined using a fluorescent inverted Leica DMIRB microscope and a UV filter (355 nm excitation and 465 nm emission).
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