Sentence examples for use a sucrose gradient from inspiring English sources

Their protocol is similar to our Protocol C, in that they use PVP and β-mercaptoethanol for prevention of polyphenol oxidation, it differs because they use a sucrose gradient for nuclei isolation and silica column for DNA isolation.

The SEC weight molecular size distributions indicate that the extraction procedure with protease is most effective in obtaining pure phytoglycogen from grain, whereas that without protease at cold temperature followed by purification using a sucrose gradient is more effective for leaf material.

Furthermore, the ease of separation of AuNPs using a sucrose gradient centrifugation is an improvement to research using biological methods for nanoparticle fabrication.

In order to find out, we used a sucrose gradient protocol, followed by hypotonic lysis of synaptosomes to prepare synaptic membranes and synaptic vesicles [20].

Exosomes were purified using a sucrose gradient where, the 100,000g pellet was re-suspended in 0.5 ml of 0.25 M sucrose (20 mM HEPES/NaOH, pH 7.2) and layered on top of a linear sucrose gradient (0.25 2.0 M sucrose, 20 mM Hepes/NaOH, pH 7.2).

Mitochondria and MAM were purified using a sucrose gradient.

Cysts were purified from feces using a sucrose gradient centrifugation as in [ 42].

Native chromatin was isolated using a sucrose gradient from HEK 293T cells.

Nuclei were isolated using a sucrose gradient protocol modified from Gendrel et al. [ 15] as described in Table 1.

Eyes were cryoprotected using a sucrose gradient (10 30% sucrose in PBS), and subsequently 12 µm sections were cut using a cryostat.

SKOV3 secreted vesicles are constituted by exosomes and apoptotic vesicles, which can be fractionated by using a sucrose gradient, as previously described [ 21, 22].