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Immediately prior to use, a stock solution of dimethylthiazolyl-diphenyl-tetrazolium bromide (MTT; Sigma-Aldrich, 1 mg/ml) in PBS was prepared.
For in vivo use, a stock solution with 0.5% methylcellulose and 0.1%Tween800 (Sigma-Aldrich, Munich, Germany) was prepared and further dissolved with water according to the manufacturer's protocol.
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We used constant total concentrations of F (379 μM; 7.2 mg L-1; using a stock solution of 37.9 mM NaF) and of Al (10.4 μM; 281 μg L-1; using a stock solution of 1 mM Al in 4% HNO3) throughout.
The levels of lipid hydroperoxides were quantified using a stock solution of t-butyl hydroperoxide.
LLE and liquid-liquid microextraction (LLME) have been compared using a stock solution of reference volatile compounds.
Crystallization was conducted in hanging drops using a stock solution of a premade complex containing Ire1KR32 (12 mg/mL) and 0.6 mmol 2'-deoxy-CCGCAG.
The experiment used a stock solution with 0.2 m of yield-determining 2 a, compound 1 a (1.1 equiv), along with [Pd(O2CCF3 2] (2 %), compound 4 (3 %), and TFA (1 equiv) in N-methylpyrrolidinone (NMP).
We used a stock solution of 200 mg BBP/ml sesame oil, and dams were gastric intubated with 2,5 μl BBP/g BW (0.5 ml for a dam of 200 g) each day, preferable each late afternoon.
The doxorubicin efficiency and loading in the nanoparticles was determined by measuring the absorbance of known amount of sample at 485 nm corresponding to λ max of doxorubicin and comparing the values to a standard curve obtained by using a stock solution of doxorubicin.
When the ODC was measured using a stock solution of compound 1 prepared in DMSO two or more months prior to mixing with hemoglobin, the P50 was 18 ± 0.1 (120 μM compound 1) and 10 ± 0.5 Torr (2 mM compound 1, P < 0.001 vs in the absence of compound 1).
This SS solution was used as a stock solution.
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