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For the present time it seems wise to use a single array type for the evaluation of microarray data.
While this information indicates that it is suitable to use a single array hybridization per mutant, replicates are recommended for the wild type line to ensure data consistency and allow for error rate examination based on wild type by wild type comparisons.
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Moreover, we opted to continue using a single array for each memory load for much of the same reasons.
To determine the efficiency and to reduce costs associated with CGH-based deletion discovery, we used a single array per deletion experiment without replicate hybridizations.
The expression levels for each individual F1 tree were analyzed using a single array only; however, analyses were conducted using pooled data from trees with similar phenotypes, which represented pseudo-replicates in this context [ 20].
Inconsistencies between results generated using different microarray platforms [ 8, 10, 11] or generations of array [ 12, 13] have been highlighted and multiplicative, systematic biases have been shown to be introduced at many stages of the experimental process, even when using a single array platform [ 12].
In this study, we investigated rare CNVs (frequency <1%) in deoxyribonucleic acid (DNA) samples from 6808 T1D cases, 9954 controls and 2206 families genotyped with ImmunoChip, which is one of the largest CNV studies to date using a single array platform.
In contrast to our previous multi-array model, we here use a single-array model to summarize the probes.
Agilent microarrays use a single-color array protocol and combine a direct labelling method with innovatively designed, in situ-synthesized probes that have minimal sequence bias [ 3].
The permanent network of Soultz-sous-Forêts can be used as a single array for periods between 2 and 5 s.
In other words, the method is robust in simple situations and with the use of a single array; however, it generally fails when distributed arrays are used, and when early reflections and head orientation issues are to be addressed.
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