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Use a sharp needle.
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Siliques were dissected using a sharp needle and seeds were extracted and transferred onto microscopic slides into the drop of the Hoyer's solution (http://www.seedgenes.org/Tutorial.html) and cleared for 1 h similarly as described (Friml et al., 2003).
Immunofluorescence of muscle myosin on embryos or adults was performed by freeze crack method as previously described (Wang et al., 2011) with the following adaptation for staining of adults: each mother was cut open in the middle using a sharp needle.
Use a sharp razor.
Use a sharp knife.
Use a sharp pencil.
Use a sharp, new razor.
Use a sharp, heavy knife.
Use a sharp object instead.
Use a sharp kitchen knife.
Always use a sharp knife.
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