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Therefore, for a definitive and reliable result using the Rio method, it is important in some cases to also use a second sample from the convalescence phase.
As the study also evaluated the invariance of the instrument for independent samples, it was necessary to use a second sample of the same size.
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The next step was to use a second (sampled) population to evaluate effects of sampling the targeted population.
A good reproducibility was found when using a second sample, resulting in −97 kJ/mol.
Validation using a second sample set showed the sensitivity and specificity to be 88% and 93%, respectively.
The majority thought that analytic validity should be confirmed using a second sample from the participant (n = 53, 72%), although some felt that the same sample could be used (n = 20, 27%).
Changes in IgG anti-HEV serologic status occurring in 2004 were determined by using a second sample taken 12 months later from a subpopulation of 3,431 participants (Table 3) from 7 of the 8 communities.
To assess the robustness of the selection of 'risk' factors identified from exploratory multivariable models using baseline data (t = 0), confirmatory analyses using a second sample was used to confirm the significance and independence of those 'risk' factors identified within a multivariable model.
The first run uses a first sample; then, any reads mapping to k-mer contigs belonging to repeats ("inchworm" contigs; see Trinity manual) are added to a second independent sample, and Trinity is performed one more time.
We first describe strategies in which a single sample is typed for markers in one or two stages, with neither the use of a second sample nor any attempt at replication of a linkage result.
A second weighting scheme was also evaluated (Table 2, weighting 2) using a second random sample of 25 patients in HOS with available information who were not included in the first sample and who did not have a prenatal diagnosis or family history of Hunter syndrome.
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