Because we wanted a rapid, robust, high-throughput and highly sensitive assay, we chose to use a quantitative real-time PCR assay instead of pyrosequencing to measure LINE-1 methylation.
The extracted DNA from whole blood was tested for the Nc-5 gene fragment of Neospora caninum using a quantitative real time polymerase chain reaction.
Study design: B19-specific antibodies were determined by ELISA and viral load was assessed using a quantitative real time B19 PCR.
To confirm our microarray results, we used a quantitative real time polymerase chain reaction (Q-RT-PCR).
However, using a quantitative real time PCR analysis H2K and B2m transcripts were detected in all pluripotent stem cell lines.
We tested this hypothesis by assaying the mitochondrial genome content using a quantitative real time PCR approach and found that it was false.
For this, the expression of 377 miRNAs was investigated using a quantitative real time PCR array technology in placentas of these three origins as well as donor fibroblast cells used for SCNT.
Presence of PCV2 in individual serum samples was measured using a quantitative real time PCR assay previously described [ 27]., with a detection limit of 1,100 DNA copies per ml (Log 3.04).
Using a quantitative real time reverse transcription PCR assay, total BCR-ABL1 p210 transcripts were quantified in the 49 month post diagnosis sample for patient three (P3) and a wild type sample at diagnosis.
After infection or addition of the inhibitor, DNA from three individually infected and 5-AZA treated cultures was extracted and bacterial load was determined using a quantitative real time PCR assay comparing A. phagocytophilum msp2/p44 normalized to a human ACTB standard.
Using a quantitative real time PCR-based assay, we found that 28% of samples in our group showed Aurora A gene amplification, a finding which was superior to the result of Zhou's research which showed amplification in 12% of primary breast cancer cell lines [ 22].
