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Due to the transient nature of ROS, it is important to use a probe that does not accumulate over time, but reflects both the rapid production and decay of ROS in the system.
We use a probe that we can move along the fiber and which we can track in three-dimensional space with nanometer accuracy.
Most of these probes fluoresce green, and so to view a second signal that occurs in the cell at the same time it's easier to use a probe that fluoresces with a different color, such as red.
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Now it is set to carry out its riskiest, most intricate manoeuvre using a probe that has no rocket landing thrusters or guidance system.
Mitochondrial DNA was quantified using a probe that specifically recognizes the mitochondrial gene MtND2.
To further investigate chromosomal alterations during male meiosis in rsw4, we examined the distribution of centromeres, using a probe that recognizes pericentromeric heterochromatin in A. thaliana [36].
Northern analysis for the region using a probe that is unique to Meg8 cDNA showed that it is expressed highly in the adult mouse brain and skin (Figure 6B).
As Figure 10 shows, Northerns using a probe that would detect both Meg9 and Mirg demonstrate that Meg9/Mirg is widely expressed in many adult mouse tissues with the highest levels being in the brain, skin, and testis.
The cellular distribution of prominin-1 transcripts within the eye was investigated in both larval and juvenile axolotl by ISH using a probe that allows the detection of all potential splice variants.
To localize centromeres, fluorescence in situ hybridization was conducted on chromosome spreads prepared as above using a probe that was prepared from the 180 bp centromeric repeat sequence (CEN), which was PCR-amplified from the pAL1 clone and labeled using the Fluorescein-High Prime DNA labeling Kit (Roche, Indianapolis, IN), as previously described [43].
Northern blot analysis using a probe that does not distinguish between endogenous wild-type tmRNA and the ssrA-DD derivative, demonstrated that the total steady state amount of ssrA tmRNA was approximately doubled in cells with a single extra-copy and was substantially higher in cells transformed with the multi-copy plasmid (Figure 1B).
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