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As Schmidt et al. [1] did not use a positive control (a substance which is known to be toxic to A. bipunctata) it is impossible to affirm that the larvae had ingested Bt toxin at all.
Some approaches use a positive control or both positive and negative controls [ 7], others do not use a control, including Z score/robust Z score and B score [ 8].
However, considering standardization of Cy3 and Cy5 fluorescence intensity as well as quality control, we use a positive control (fully-methylated APC allele from clone) just like the housekeeping gene of cDNA microarray and a negative control which removes the influence of cross-hybridization.
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Finally, we calibrated absorbance values between plates using a positive control.
RNA isolated from murine brain endothelial cell line (bEnd.3) was used a positive control.
The strain SMGS5-PnlmAB was used a positive control.
Also, we used a positive control for the analysis a sample of Mycobacterium (M.
Calibration of the test was achieved using a positive control sample.
For Ki-67 and cleaved caspase-3, human tonsil tissues were used a positive control.
Staining development was performed with DAB with timed monitoring using a positive control sample.
N. fowleri Lee ATCC 30894 DNA was used a positive control in the PCR reactions.
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