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When the source of the variability is not reproducible, or the effect is uncontrollable, then it is necessary to use a nested design.
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For example, in studying the cases of autism, researchers might use a nested case control design in which a large screening sample is used to identify the cases and a subsample of the non-case sample members is selected for the control sample.
A total of 420 tadpoles (140 of each genotype) were used in a nested design (host genotypes nested in host species) allowing us to discriminate the effect of host species versus host genotypes in driving infection outcomes along a gradient of genetic distances (phylogenetic versus genotypic).
We will use a nested case-control study design to identify 1000 women who developed PTSD following trauma exposure and 1000 controls that experienced similar traumas but did not develop PTSD.
We use a nested optimization strategy to find the optimal plant dynamics and control effort for the transition.
To look at longer time scales, we use a nested spectral analysis method.
Using a hierarchical nested design, we measured quarterly nine physiological traits, and monthly ten morphological traits from 9720 individuals at 27 plots along water depth gradients in Lake Erhai, China.
A nested design was used, such that 25 physicians each saw six patients, two patients in each of three communication skills interventions (i.e. trained, informed, control).
A nested design was used in order to assess differences between species across their vertical zonation and to compare the effects of zone only within each species.
A nested design was used to match patients who initiated warfarin after REVEAL Registry enrollment to patients never on warfarin within each etiologic cohort (IPAH and SSc-PAH).
This difference was not significant if a nested design (ploidy, population, mother plant) was used but significant if the population level was not considered, indicating high interpopulation variation in this trait.
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