Here we investigate the relationship between regionally specific sleep spindle activity and motor memory consolidation, using a nap paradigm, incorporating the above described motor-sequence task.
Using a nap paradigm, here we demonstrate the selective offline benefit of sleep on the consolidation of negative emotional memories.
Following overnight incubation at 4°C, the excess dye was separated from the labeled proteins using a NAP desalting column (GE Healthcare).
Using a nap paradigm, we measured the consolidation of neutral and negative emotional memories, and the association with REM-sleep electrophysiology.
Using a nap paradigm, here we explore the consolidation of neutral and negative emotional memories, and test the hypothesis that affective memories are selectively facilitated by REM sleep, and specifically oscillations in the theta frequency range.
Fractions were analysed by SDS/PAGE, and Sirt1 fractions were pooled and transferred into storage buffer (25 mM HEPES, pH 7.5, 100 mM KCl and 2 mM DTT) using a NAP column (GE Healthcare), aliquoted, snap-frozen in liquid nitrogen and stored at −80°C.
Subsequently, the buffer was changed to 0.1 M citrate, pH 5.5, using a NAP-5 column (GE Healthcare, Buckinghamshire, UK).
Excess ligands were removed and buffer was exchanged into 0.25 M CH3COONH4 (pH 5.5) using a NAP-5 column (GE Healthcare, Glattbrugg, Switzerland).
Labelled Fab fragment was then separated from the non-reacted radionuclide and low-molecular-weight reaction components by using a NAP-5 column pre-equilibrated with PBS.
