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We use a modified protocol to isolate primary OC cells from ascites that involves a single step to reliably produce OC cultures.
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The NMD analysis was performed using a modified protocol described by [19].
Pigs were anesthetized using a modified protocol described by Goldmann et al. [ 19].
Total cellular RNA was extracted using a modified protocol described elsewhere (Fox et al., 2009).
Cleared whole mounts were prepared using a modified protocol described by Wellings et al [ 9].
Flow cytometric evaluation of the cell cycle was performed using a modified protocol (Amant et al, 2003).
Oil Red O staining was determined using a modified protocol described by Ramírez-Zacarías JL et al. [ 18].
We have used a modified protocol to extract DNA from a complete blood spot in a single reaction (see supplementary material for details).
The cDNA is end-repaired and A-tailed, and diluted TruSeq adapters with barcodes are ligated using a modified protocol (Illumina) [ 23].
DNA was extracted from all I. scapularis nymphs with QIAGEN DNeasy blood and tissue kit (QIAGEN Inc., Valencia, CA, USA) by using a modified protocol (19 ).
Total RNA was isolated from 200 μL of rat urine using a modified protocol (miRNeasy RNA extraction kit, Qiagen) according to the manufacturer's instructions.
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