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We also use a minimum threshold for the total calling time in the entire period, (t_{mathrm{min}}=30) minutes.
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For example, using a minimum threshold score of ≥0.8, 98% of all CDS are members of the most conserved gene families (above the division-level).
We used a minimum threshold of E-value < 1E-03 to consider hits for further analysis.
Binning was constrained using a minimum threshold on percent identity for alignments that met the default maximum E-value.
Using a minimum threshold of 0.2, we identified among all 230 loci, 43 most discriminating AFLP loci.
Convergence to the stationary distribution of the single chains was inspected using a minimum threshold for the effective sample size.
Additionally, genes with consistently low expressing signal across all ten developmental stages were removed using a minimum threshold of 0.712, empirically decided using GeneSpring's Cross Gene Error Model.
To minimize lifetime calculation errors, we used a minimum threshold of 15 photons at the peak (corresponding to ∼1000 photons per pixel) and a binning factor between 2 and 10 pixels to ensure sufficient photons in the regions of interest.
Using a minimum threshold of 7× coverage, we recovered approximately 90% of V1R I sequences previously identified in the draft M. murinus genome (59% being identical at all nucleotide positions).
The SC method was run using a minimum threshold value of 0.2501 for full-sibling families with two members, and 0.1667 for full-sibling families with three or more members.
To address the sequencing depth variation of the markers, we normalized them using a minimum threshold (set as 5000× for the salt response trait and 3000× for the seed size trait).
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