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In contrast, eukaryotic hyaluronidases use a hydrolytic mechanism; this reaction is not accompanied by a spectrophotometric change.
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LiHA scaffolds were degraded via a hydrolytic mechanism when they were soaked in SBF.
This review focuses on research undertaken over the past few decades which has sought to mimic the hydrolytic action of metal-containing nucleases with synthetic transition metal complexes that cleave through a hydrolytic mechanism.
It is therefore unlikely that the substrate specificity of DEBS AT3 is determined by a hydrolytic mechanism, but rather likely that the enhanced hydrolytic rate simply reflects the strong substrate specificity of this cis-AT domain.
This is a target for an AP endonuclease (APE1), which cleaves the phosphodiester backbone on the 5′ side of the AP site via a hydrolytic mechanism.
It was thought that the loss of chloride by elimination or by a hydrolytic mechanism could possibly lead to either vinylating or hydroxyethylating species, respectively.
Use a stress release mechanism.
In contrast to typical cellulases, which are hydrolytic enzymes, LPMOs are metalloenzymes that degrade cellulose using a mechanism involving molecular oxygen and an electron donor [ 3, 4].
We now know that HDACs can act by a simple hydrolytic mechanism (the classical HDACs, which contain a crucial zinc ion), or a complex NAD-requiring mechanism (the sirtuins).
Family 29 enzymes use a two-step double-displacement mechanism for the catalysis, (retaining enzymes i.e. the anomeric configuration of the released product is the same as in the originally cleaved molecule), while glycosidases from family 95 are among so called inverting enzymes catalyzing the hydrolytic process by direct displacement mechanism.
The best Fe3O4 nanoparticles (NPs) in terms of SAR values have been synthesized using a non-hydrolytic thermal decomposition method [15 17].
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