As a final step, antibody specificity was confirmed by using a highly specific MERS-CoV microneutralization assay as described (6 ).
This may not be valid using a highly specific cortisol immunoassay or liquid chromatography-mass spectroscopy (LCMS/MS).
The immunofluorescent method is free of such limitations, as it depends entirely on detecting the receptor using a highly specific monoclonal antibody.
Thus, the present study was designed to determine the detailed localization of 5αR2 in the adult rat brain, using a highly specific polyclonal antibody against this isoform.
We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag.
Plasma LH level was assayed using a highly specific rabbit anti-serum, anti-native rat LH (ALPCO diagnostics # 29-R002) wI125I125 as tracer.
Plasma corticosterone levels were measured by radioimmunoassay (RIA) as described elsewhere [19] using a highly specific corticosterone antiserum (MP Biomedicals, France).
To determine whether ATM plays a role in the resolution of IRIF in hESCs, NPs, and astrocytes, we used a highly specific ATM kinase inhibitor, KU-55933 [23], [24], [24].
In the current study we rigorously compared whole cell lysates from wild type, ΔCS, and FasL KO mice for FasL expression using a highly specific anti-peptide rabbit antiserum for Western blot analysis [24].
