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The retrotranscriptase reaction was carried out using a High Capacity cDNA Archive kit (Applied Biosystems, US) following the protocol provided by the supplier.
cDNA was synthesized using a high capacity cDNA archive kit (Applied Biosystems).
cDNA synthesis was performed with 500 ng of total RNA using a High Capacity cDNA Archive Kit (Applied Biosystems).
10µg of total RNA was reverse transcribed in a 100µl reaction using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) according to manufacturer's instructions.
Total RNA (2 µg) was reverse-transcribed to cDNA in a 20 µl reaction volume using a high capacity cDNA reverse transcription kit (Applied Biosystems).
An aliquot of 2ug RNA were arrayed in 96-well plates and then converted to cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
After treatment with DNase (Turbo DNA-free Kit, Ambion, Austin, TX), RNA was reverse transcribed to cDNA using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA).
One hundred nanograms of RNA were reverse transcribed to cDNA using a High Capacity cDNA (Applied Biosystem).
RNA was reverse transcribed using a high capacity reverse transcription kit (Applied Biosystems).
cDNA synthesis was performed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Warrington).
cDNA was made using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
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