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For example, up until a few weeks ago, I didn't know how to use a french press.
I prepare loose-leaf tea in a Rishi glass teapot but you could use a French press.
Even if he weren't so funny, we have to admit: This is just a good instructional video on how to use a French press.
#4 -- Prepare "Titanium Tea" (this name was a joke, but it stuck) (two to three minutes) I prepare loose-leaf tea in a Rishi glass teapot but you could use a French press.
Coffee drinkers were instructed to use a French press or ceramic drip in place of a plastic coffee maker.
See How to use a French Press for more details.
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Harvested cells were resuspended in buffer, broken using a French press and incubated at 80°C for 20 minutes.
The harvested cells were disrupted by using a French press (Aminco, Maryland, USA) and the cell debris was removed by ultracentrifugation (100,000 × g, 30 min, 4°C).
After breaking the cells by using a French press, the unbroken cells were removed by centrifugation at 14,000 rpm for 20 min.
The cells were pelleted, washed twice with ice-cold PBS buffer (101.4 mmol/L Na2HPO4, 1.8 mmol/L KH2PO4, 137 mmol/L NaCl, 2.7 mmol/L KCl, 8% glycerol, pH7.4), dissolved in the same buffer and lysed using a French Press.
Lyophilizing harvested cells prior to lysis using a French press, bead beater, mortar and pestle, or ultrasonication resulted in large (up to 6-fold) increases in soluble protein concentration (up to 60 mg/gFW) and glycerol kinase activity (up to 2 μmol/min gFW− 1) in the resultant clarified extracts.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com