In our studies, we counted colonies of 50 or more cells manually using a low-power stereoscope, and we documented images of each plate using a digital imaging system.
The slides were examined using a digital imaging microscope (Zeiss, Germany).
These protein bands were quantified using a digital imaging system (UVtec, Cambridge, UK).
Photographs were taken using a digital imaging system (Funakoshi, Tokyo, Japan) incorporating an inverted digital camera (Canon, Tokyo, Japan).
The chest x-rays were obtained in anteroposterior direction with the patient in supine position using a digital imaging system.
Images obtained using a digital imaging system (ImagePro 3.0, Media Cybernetics Inc., Silver Spring, MD, USA) connected to a microscope were coded and analyzed in a masked fashion.
Nonstaining bands representing the levels of the latent forms of MMP-2 and MMP-9 were quantified by densitometer measurement using a digital imaging analysis system.
Membranes were exposed to X-ray film and film developed, spots were quantified by densitometry using a digital imaging system (Image Lab software, Berkeley, CA, USA).
Immediately after this, the chemoluminescent agent was removed and the PVDF membrane was analyzed using a digital imaging system (G Box, Syngene, MD, USA).
Conventional, wide-field fluorescence imaging was performed using a digital imaging system (Olympus BX-FLA) attached to an upright microscope (Olympus BX50WI, 40x water immersion objective) and a CCD camera as sensor (Princeton Micromax, Princeton Instruments, Trenton, NJ, USA).
