NK cells were isolated by negative selection from PBMCs depleted of monocytes using a depletion cocktail of antibodies directed to CD3, CD4, CD14, CD19, CD20, CD36, CD123, CD66b, Glycophorin A (StemCell Technologies).
Dissolved organic carbon water partition coefficients (KDOC) for wastewater derived and reference DOC were measured for a range of micropollutants using a depletion method with polydimethylsiloxane disks.
In this study, we used a depletion method before MS and labeling by isobaric tag for relative and absolute quantitation (iTRAQ).
That study contrasts with ours in that we used a depletion method that selectively removed the most abundant serum proteins, thus allowing us to examine the full range of proteins.
These were obtained from a pure gas phase model and gas grain interactions were not directly included in this calculation other than using a depletion parameter which decreases the relative elemental abundances of the heavier species such as O, N, and C toward clouds with higher densities.
Infected-erythrocytes were purified by magnetic isolation using a MACS depletion column as described above.
To obtain synchronous asexual stages, parasites were synchronized by the isolation of trophozoites by magnetic isolation using a MACS depletion column (Miltenyi Biotec) in conjunction with a magnetic separator, and placed back into culture.
NK cells were enriched from PBMCs using a CD3+ depletion kit (Miltenyibiotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions.
Using a negative depletion technique we were capable of isolating CTC, without using a priori selection markers.
In contrast to PALM and dSTORM, STED microscopy uses a specialized depletion laser, in combination with the excitation light.
