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It is also possible to use a column of cationite and zinc amalgam, using 1 M hydrochloric acid as an eluant, reducing Md III) to Md II) where it behaves like the alkaline earth metals.
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The hydrodynamic characteristics of the CDC were investigated using a column of 0.076 m (3in) i.d. with liquids of different surface tension, viscosity and coalescence properties.
This was demonstrated by assembling a 4 × 4 matrix of 20-μm-diameter polystyrene beads using a column of four OFB microrobots (Figure 5).
This enables each frame (in our case each I-Frame) to be capped to the same size by using a column of intra blocks that move across the video trace from one side to the other, thereby "refreshing" the image.
The native molecular weight of MIF-II was estimated by using a column of Sephacryl S-300 (1×60 cm) by modification of the method of Wollny et al. [34].
DHP-PMX was performed using a column of polystyrene fibers to which polymyxin B was covalently bound at a weight ratio of 0.5%.
The molecular masses of IMAC purified β-xylosidase complexes were determined by SEC according to Michlmayr et al. (2013) using a column of 110 mL.
The recombinant proteins were purified from cytosolic fractions by affinity chromatography as described above using a column of Ni-NTA resin.
Samples (1.4 ml) were collected immediately after pretreatment and filtered using a column of Dowex 1 × 2 to eliminate the H2SO4 from the samples.
Antibodies were affinity purified from the terminal bleed serum using a column of SNX51 35 peptide conjugated to SulfoLink Coupling Gel (Pierce , USA according to manufacturer's instructions.
The PCDD/F and dl-PCB fraction was isolated and a clean-up was performed using a column of AgNO3 on silica gel and a column of activated Al2O3 on silica gel.
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