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We use a buffer solution (triethanolamine hydrochloride) as aqueous and MtBE (tert-butyl methyl ether) as organic phase which provide good enzyme compatibility.
Why, then, not use a buffer solution instead of saline?
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Chemical sensor was then regenerated using a buffer solution at pH 2.0 before being dipped into permethrin solution with the same concentration.
Ngo et al. (2011) improved the hydrogen yield (1.55 2 fold) compared with the un-supplemented culture by using a buffer solution (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) and N2 sparging conditions (Ngo et al. 2011).
The pH was measured using a pH meter (Mettler-Toledo, England) calibrated using a buffer solution (Merck, KGaA, Darmstadt, Germany) at pH 4 and 7.
For detergent exchange in purified MG23 preparations, gel filtration chromatography was performed using a buffer solution containing 16 mM CHAPS and 16 mM 1,2-dimyristoyl sn-glycero-3-phosphocholine (DMPC).
Cells were lysed using a buffer solution containing 25 mM TrisCl, 1 mM EDTA, 5 mM MgCl 2, 1 mM DTT, 100 mM NaCl, 10% glycerol, 1% Triton ×-100, 1 mM PMSF, and 5 μg/ml each of leupeptin, aprotinin, and pepstatin.
Sodium acetate (50 mM) was used as a buffer solution.
The CTAB coating on the surface of FeCo nanoparticles is an antiseptic agent against bacteria and fungi and is used as a buffer solution for the extraction of DNA.
The ovaries were softened in a 4 N sodium hydroxide solution at 60 °C and stained by use of a buffer solution containing 0.1% water-soluble aniline blue dye dissolved in 0.1 N K3PO4.
Purified mRNA was sheared to 200- to 600-nt fragments using a buffered zinc solution (RNA Fragmentation Reagent; Ambion).
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