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Synonymous codon usage order (SCUO) was obtained from the CodonO platform [ 35].
Synonymous codon usage order (SCUO) analysis was performed online using the CondonO platform [ 35].
We designate the statistics O i as the synonymous codon usage order (SCUO) for the i-th amino acid in each sequence.
SCUO: Synonymous Codon Usage Order GC1: The GC composition for the first site of each codon GC2: The GC composition for the second site of each codon GC3: The GC composition for the third site of each codon XFW was responsible for project design and implementation, software implementation, and manuscript writing.
We presented an analytical model to quantify the non-linear relationship between GC3 and a measurement of codon usage bias (synonymous codon usage order, or SCUO), which reveals that GC3 is the key factor driving synonymous codon usage and that this mechanism is independent of species.
Translation efficiency is also known to be affected by codon usage, so we included synonymous codon usage order (SCUO), which was obtained from the CodonO platform [ 35]; higher values indicate more codon bias, meaning less random codon use in a gene's coding region.
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