Sentence examples for usage of the native from inspiring English sources

The sequence of each group in Table 5 reflects the degree of the usage of the native language, the popularity of the common national language (Mandarin) in the 18 ethnic minorities, and the degree of mixed usage of ethnic languages.

The improved expression of FtsZ and P38α from synthetic genes in E. coli may result from the biased codon usage of the native genes leading to abortive heterologous protein expression when rare tRNA pools become depleted in vivo.

A cDNA sequence encoding the swapped version of histone H1.1 was synthesized using the codon usage of the native form and was bacterially expressed and purified as shown in the inset of Figure 2. The proteins were then re-natured using a previously described protocol from our laboratory [ 21, 25] and their secondary structure organization was determined by CD.

Adaptation of the codon usage signature of the native gene on the expression host rather than synonymous substitutions to frequent codons can significantly improve the recombinant expression [50].

However, the bias of codon usage between the native gene sequence and P. pastoris has significant impact on the expression level of recombinant protein.

It has now been shown that the difference of codon usage between the native gene sequence and expression host has significant impact on the expression level of recombinant protein [ 14, 15].

Due to the difference of codon usage between the native gene sequence and expression host, researchers have used codon optimization to increase the expression level of heterologous genes in P. pastoris.

In this approach, two features are primarily considered: first, that the expression host synonymous codon usage should more closely match that of the native gene host codon usage, and second, that putative nonstructural segments between local alpha-helical content are coded to translate more slowly.

Guatemala does not have legal regulations on the usage of native inhabitants DNA pool (according to the Instituto Nacional de Ciencias Forenses during the sample period; INACIF; http://www.inacif.gob.gt/).

However, recombinant expression of foreign proteins is typically suboptimal possibly due to the usage of non-native codon patterns within the coding sequence.

Each codon-shuffled sequence strictly retained the codon usage frequencies of the corresponding native sequence.