Sentence examples for usage of a cryptic from inspiring English sources

The insertion of 33 nucleotides upstream of exon 11 in Csn1s1 SEG/Pas is likely due to the usage of a cryptic splice site.

RT-PCR analysis and sequencing revealed a smaller amount (21 ± 6%) of an alternative transcript that originates from the usage of a cryptic 5′ss located 104 bp upstream of the authentic 5′ss (Fig.  1B).

Sequencing of RT-PCR products from the mutant revealed that this causes two types of abnormal products: the usage of a cryptic splice donor distal to exon 7 and skipping of exon 7. The first form ("MutA") introduces foreign amino acid codons from intron 7 sequences, causing a premature stop upstream of the IQ motif at the C terminus of the protein.

In this study, the c.5074G>A/p.Asp1692Asn, c.5074G>C/p.Asp1692His, and c.5074G>T/p.Asp1692Tyr variants were shown to induce skipping of exon 17 as well as usage of a cryptic splice donor site located at c.5074 + 153 in intron 17.

This large deletion is due to the usage of a cryptic splice site occurring in exon 21, 57 nucleotides downstream from an AG defining the proper end of intron 20, in frame and following a rather strong polypyrimidine tract (n = 20) although interrupted by a triplet of contiguous G.

This substitution was predicted to be pathogenic by abolishing the conserved donor splice site (Mutation Taster; Human Splicing Finder v2.4.1; NN SPLICE) [ 2], with potential usage of a cryptic donor splice site present 294 bp downstream in intron 5 (0.88 NN SPLICE).

In previous research, modifications of GFP by optimization of codon usage and elimination of a cryptic intron can greatly enhance the GFP expression in plants (Chiu et al. [1996]; Haseloff et al. [1997]).

We suspect that an second lariat branch point was used to produce the correctly-spliced fraction of BAX mRNA, perhaps sites A or B. Similar usage of a back-up, cryptic branch point sequence has been seen in the case of the β-globin gene [32].

Three of them almost abolished the usage of the immediate downstream (proximal) 3′AG and activated substantial usage of a further downstream cryptic 3′SS within the exon or caused exon skipping (upper right, lanes 3, 5 and 7).

First, we demonstrate that several mutations at the exon 2 5′ss of coagulation F9 gene produce aberrant splicing by inducing the usage of a strong exonic cryptic 5′ss, which is regulated by adjacent exonic splicing regulatory elements, both ESE and ESS.

In particular, by exploiting the F9 exon 2 context as study model, we demonstrated that both mutation types, through distinct mechanisms, shift the balance toward the usage of a strong exonic cryptic 5′ss.