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This effect is possibly caused by the significantly greater density of the usage matrix
In contrast, Jiao et al. (2014) and Malikic et al. (2015) try to identify the usage matrix and clonal tree simultaneously.
Thus the covariance matrix for the predicted codon usage matrix Y ^ is (n denotes the row number) (9) M = 1 n − 1 Y ^ T Y ^.
As AncesTree directly computes the usage matrix U, we obtain estimates of the amount of mixing, or intra-tumor heterogeneity, of clones within each analyzed sample.
To optimize under these conditions, a codon usage matrix was calculated using the 50 genes most differentially upregulated when comparing three day cultures to exponential growth [ 38].
D efinition 3 An m × n matrix U = [ u p j ] is a usage matrix provided u p j ≥ 0 and ∑ j = 1 n u p j ≤ 1.
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First, XopD N-terminal extension presents a low coding probability using standard codon usage matrices.
Let U m, n be the set of all m × n usage matrices U.
Using those corresponding gene target sequences, codon usage matrices were constructed for each transcription factor.
Codon usage matrices for the gene targets regulated by each of these global regulators were compared with the control matrix derived from all 6,666 protein coding genes (see Additional file 3 for all matrices).
The remaining transcript assemblies were translated by ESTScan3 (27) using appropriate custom built codon usage matrices from either A. thaliana, O. sativa or a combined matrix of Pinaceae species: Piceae sitchensis, Picea glauca and P. taeda.
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