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We performed bioinformatic analyses of fully annotated ORFs to evaluate the codon usage in sequences of consecutive lysines found in the E. coli and S. cerevisiae transcriptomes.
Codon usage of all C. albicans genes was derived from the Candida Genome Database [ 24] and codon usage in sequences of ORFs bound by Tpk2 were calculated using the Codon Usage Calculator [ 43].
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Contrary to what has been reported for subtype B, the optimal approaches for prediction of CXCR4-usage in sequence from viruses that use CXCR4 exclusively, also perform best at predicting CXCR4-use in dual-tropic viral variants.
We also report that in HIV-1 group M subtype C, sequences from dual-tropic viruses have minimal effect on accuracy calculations and the optimal approaches for prediction of CXCR4-usage in sequence from viruses that use CXCR4 exclusively also perform best at predicting CXCR4-use in dual-tropic viral variants.
Similar results were observed when considering the ratio of nucleosome favoring (GC, CG, GG) and disfavoring (AA, CA, AC) di-nucleotide usage in the promoter sequences of these two groups of genes.
Identification of the TRDD genes within the bovine genome allowed us to evaluate TRDD gene usage in cDNA sequences that had been previously sequenced in our laboratory.
However, codon usage bias in the sequences of the Group 1 Tc1 elements showed a unique pattern, which was different from those of both lampreys and teleost fishes (data not shown).
Furthermore, we are aware of the finding of Lobry and Necsulea [ 40] that thermophile-mesophile signatures of cold adaptation are more prominent [ 43] than mesophile-psychrophile signatures, at least in analyses of codon-usage trends in coding sequences of complete genomes.
Therefore, we compared overall C. albicans codon usage with codon usage in ORF sequences bound by Tpk2.
In this study we altered the codon usage in the signal sequence of the bla gene, encoding β-lactamase in Escherichia coli.
In this chi-square analysis, the null hypothesis specified that nucleotide usage in 50 nt of exon sequence upstream and downstream of the different intron insertion sites was random and dependent on the nucleotide composition of Euascomycetes SSU and LSU rRNA sequences in general.
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