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Also, the species studied showed similar patterns of AS usage in genes of different evolutionary age.
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This is so because the evolution of an organism leads to an optimization of the codon usage in genes for the specific physiology of that organism [ 29].
There are usually no distinct strand-specific divergences in nucleotide usages in genes of Trend III or Trend V organisms (data not shown).
In an attempt to investigate whether the strand-specific mutational bias has any impact on amino acid usage in gene products of LL strains of P. marinus in comparison to their HL counterparts, we performed correspondence analysis (COA) on relative amino acid usage (RAAU) of the encoded proteins of each organism.
Currently, the more popular view is that codon usage in genes should reflect the availabilities of tRNAs when these genes are expressed most actively.
Based on the results from the ΔRSCU analysis, I identified 25 codons from 18 different amino acids that were used to calculate the frequency of optimal codon usage in all genes of the different species.
So there is a need to better understand the information content of biased codon usage in genes and to identify a mechanistic link between codon-usage patterns and specific tRNA modifications.
Currently, few data are available about the genomic traits and evolution of N. tetrasperma and N. discreta, including the evolution of codon usage in gene coding DNA.
The effective number of codons (Nc) quantifies how far the codon usage of a gene departs from equal usage of synonymous codons (Fuglsang 2004) and is a measure of codon usage biases in genes and genomes that ranges from 20 (maximal bias) to 61 (unbiased) (Wright 1990; Sun et al. 2013).
The patterns of codon usage in these genes, studied as the effective number of codons (Nc') and the GC content in synonymous sites (GC3), were highly similar in all study species.
Our null models make the following assumptions: fixed amino acid sequences of the DNA pol α proteins in each species; fixed codon usage bias in the genes of each species with independent evolution of codons; and fixed base composition of each intron independently with independent evolution of bases and possible splicing constraints.
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