Sentence examples for usage for expression from inspiring English sources

Further sequences encoding targeting signals have been synthesized at the GeneArt Company with optimized codon usage for expression in P. tricornutum.

Accordingly, in this study we report the use of synthetic genes designed from the published amino acid sequence of the mature bacteriocins SRCAM 602, OR-7, E-760, and L-1077 and with adapted codon usage for expression by P. pastoris, their cloning into the protein expression vector pPICZ αA, and their expression by recombinant P. pastoris X-33.

An amended gene sequence encoding Nluc was designed by optimizing codon usage for expression in mammalian cells (www.kazusa.or.jp/codon), removing potentially strong mRNA secondary structure (http://mfold.rna.albany.edu), and removing consensus promoter sequences, other transcription factor binding sites, and potential eukaryotic mRNA splice sites.

Genes coding for these enzymes were synthesized into the vector pUC57 (Genescript, NJ, USA) with codon usage optimized for expression in M. extorquens AM1 in which the codon usage frequency was calculated by counting codon frequency in a list of ORFs associated with the 129 highly expressed genes in both methanol-grown and succinate-grown M. extorquens AM1 cells.

We obtained the HPyV9 VP1 coding sequence by total synthesis with a codon usage adapted for expression in Spodoptera frugiperda cells (Genscript, Piscataway, NJ, USA) (GenBank accession no. HQ696595).

Data sets were selected for general usage (preference for expression profiling of tissues over more specific sets) and date of publication (preference for recent data sets due to difficulties of mapping older sets to current gene predictions).

PCR-based de novo gene synthesis is now frequently used in molecular biology laboratories worldwide, with the free online tools to automate the primer design and, more importantly, to optimize the codon usage for efficient expression in intended organism, such as for E.coli or yeast expression [12], [20].

To help design the assembly primers, as well as to optimize codon usage for various expression systems, some dedicated computer software [19] and internet online tools [12], [20] have been developed.

Various strategies have been used to minimize the bias in codon usage for heterologous expression.

Adaptation of the E. coli-specific codon usage for GNTR expression in mammals abolished protein aggregation and enabled generation of high protein levels in COS-7 cells, as shown for other bacterial genes [ 20, 21].

In Epstein-Barr virus latent stage genes appear to deoptimize codon usage perhaps to reduce competition with host cell translation [ 25] and papillomavirus codon usage appears optimized for expression in certain cell types [ 26].