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These results were further investigated for correlations between the amino acids encoded by the trinucleotide repetitions and the codon usage preferences reported for each organism in the Codon Usage Database from the Kazuka DNA Research Institute [ 38].
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To improve the production of recombinant hIL-25 in P. pastoris, we carried out a codon optimization of the hIL-25 gene and constructed an artificial gene according to the specific codon usage of P. pastoris (codon usage database from http://www.kazusa.or.jp/codon).jp/codon
A complete and reliable HIS is essential for planning UHC; such a HIS should consider population movement, relevant to ease of obtaining access to healthcare outside of the person's residential area, and it should be possible to link health usage databases from different healthcare providers.
Codon bias tables and GC/AT percentages were calculated using the Countcodon program available from the Codon Usage Database [ 43].
The codon usage frequency and the correspondent RSCU values were acquired from the Codon Usage Database (www.kazusa.or.jp/codon).jp/codon
The codon usage of B. mori ribosome used in calculating the CAI value was also downloaded from the Codon Usage Database.
The codon usage of the 48 CDSs (12937 codons) in mtDNA and the 1180 CDSs (450043 codons) in the gDNA in Table 1 were downloaded from the Codon Usage Database (http://www.kazusa.or.jp/codon/).jp/codon/
The countcodon program from the web site of the Codon Usage Database [ 50] was used to determine absolute counts of all codons of the twenty-three rbcL sequences (396 codons).
Codon frequencies were obtained from the Codon Usage Database [ 63, 64].
For many species this data can be retrieved from the Codon Usage Database.
The codon usage data of H. sapiens and R. aegyptiacus were obtained from the Codon Usage Database [ 29].
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