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Although the changes in codon usage observed in the P. tremula lineage are consistent with an increase in the strength of natural selection acting on codon usage, an alternative explanation is a change in base composition driven by changes in mutational biases.
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The region also shows remarkable diversity within bilaterians (fig. 1 B ). First, the 10th exon is alternatively spliced in diverse bilaterian lineages, with usage of an alternative splice site within the exon.
At any rate, the usage of an alternative genetic code in Spironucleus but not Giardia [ 26] indicates that codon usage have been remodeled in the lineage leading to Spironucleus, at least for the stop and glutamine codons.
The rapid popularization of smartphones has contributed to the growth of online Instant Messaging and SMS usage as an alternative way of communication.
The MVP variant (rs13332078) promotes the usage of an alternative 3′ splice site upon stimulation with R848 (modified protein, Student's test prsQTL < 0.007), and is associated with increased risk of multiple sclerosis54.
Finally, the advantages of implementing a DSL are the improvement of the development productivity, the fact of facilitating the communication between domain expert through an explicit syntax, and above all, the usage of an alternative computational model.
Therefore, increased infectivity of HPV16 in cells with inhibited clathrin- and caveolin pathways additionally argues for usage of an alternative endocytic mechanism.
Furthermore, we have previously found that mutation of the normal splice site leads to the usage of an alternative splice donor including the GU of this fifth codon [29].
In some cases, usage of an alternative downstream promoter leaves a transcript without predicted domains.
In KIAA1217, usage of an alternative start of transcription leads to a shorter transcript that is in-frame.
To our knowledge, cancer-specific usage of an alternative start of transcription in KIAA1217 has not been reported before.
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