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Cells infected with GFP-tagged empty lentiviral vector (pGIPz) or vector encoding an shRNA against p21 (V3LHS_322234, Thermo Scientific, Pittsburgh, PA, USA) were selected using puromycin.

Households in both the UK and the USA were selected using random digit dialling, and both samples were drawn to be representative of the geographic population distribution in each country or region.

Baso-lateral membranes were selected using fluorescence microscopy (EVOSfl AMG-Life TeCAnologies, CA, USA fitted with green, red and blue filters).

Real-time probe sets were selected using the Primer Express program (Applied Biosystems, Foster City, CA, USA).

CD11b cells were selected using CD11b microbeads (Miltenyi Biotec, Auburn, California, USA), according to the manufacturer's instructions.

They were stably transfected into HL60 cells by electroporation and transfectants were selected using 2 μg/ml Blasticidin (Invivogen, San Diego, CA, USA).

Probes were selected using the ProbeFinder v2.04 software (Roche, IN, USA).

FaDu and SCC25 cells stably expressing β2M were selected using 400  μg ml−1 G418 (Calbiochem Novabiochem, San Diego, CA, USA).

Cells were selected using 1-2  μg/ml puromycin (Sigma Aldrich, St . Louis MO, USA).

Optimal primers and probes were selected using Software Primer Express Ver.1.7 provided by PE Applied Biosystems (Foster, CA, USA).

Cells expressing Wnt1 were selected using 2 mg ml−1 of zeocin (Invitrogen, Carlsbad, CA, USA).