Mice and rats were bred using standard animal husbandry.
Muscle tissues for this study were obtained from the mice used in a previous Losartan study as follows; C57BL/6J Lama2dy-2J heterozygote mice (Jackson Laboratories, Bar Harbor, ME, USA) were bred at the Hebrew University specific pathogen-free animal housing facility.
CD1 mice, purchased from Charles River Wilmingtonn, MA, USA), were bred and maintained in our animal facility according to institutional guidelines.
Nfkb2 null mice (C57BL/6) [ 6] (obtained from Christopher Hunter, University of Pennsylvania, Philadelphia, PA, USA) were bred with Eμ- Myc transgenic mice (C57Bl/6) [ 23].
Per2::luciferase mice (courtesy, Dr. Joseph Takahashi, Northwestern University, Evanston, IL, USA) [35] were bred in-house with C57/B6 (Clea Japan, Tokyo).
All animals were bred by the use of artificial insemination.
Mice were bred in the Leicester University Biomedical Services facility using animals originally obtained from either the Jackson Laboratory (Bar Harbor, ME, USA, Apc Min and TAgs) or the NCI Mouse Repository (NCI Frederick Rockville, MD, USA, TRAMP).
All experimental animals were bred for 7 d before use.
Colonies of wild-type and MIF−/− mice were bred at Charles River Wilmingtonn, MA, USA).
These cats, which were bred by Harlan (Indianapolis, IN, USA), were imported at 9 months of age.
