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Three-day-old larvae stained with an anti-TH antibody (Immunostar, Hudson, USA) were analyzed using a Zeiss Cell Observer Spinning Disc microscope.
To create the reference standards, genuine samples provided via prescription by a national pharmacy chain (West Lafayette, IN, USA) were analyzed using the Raman spectrometer and cross-checked against a second lot from a separate pharmacy to verify consistency and determine method robustness.
Blood samples from the Department of Cancer Immunobiology (Dallas, TX, USA) were analyzed using the ferrofluid assay (method A).
Data acquired on a FACSCalibur (BD Immunocytometry Systems, San Jose, CA, USA) were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).
Positive antibody binding was visualized using an ImmunoStar LD system (Wako), and protein-transferred PVDF membranes (Bio-Rad, CA, USA) were analyzed using the Lumi-imager TM chemiluminescence detection system (Roche Diagnostics, IN, USA).
Pretreatment sera from three cohorts of patients with the diagnosis of RA based on the ACR classification criteria [ 15], who were initiated on therapy with the anti-TNF therapy etanercept (Enbrel®, Amgen, Thousand Oaks, CA, USA), were analyzed using synovial antigen microarrays (except for the third cohort), ELISAs, and a multiplex 12-cytokine bead assay.
Geological deformation in the northern New Madrid seismic zone, near Olmsted, Illinois (USA), is analyzed using integrated compressional-wave (P) and horizontally polarized-wave (SH) seismic reflection and regional and dedicated borehole information.
Images were captured by GelDocTM XR+ system (Bio-Rad, USA) and were analyzed using PDQuestTM 2-D Analysis Software (BioRad, USA) for MW and pI calculation.
Data were entered into an electronic data base using an optical form scanning system (TeleForm version 7.1 Cardiff, Vista CA, USA) and were analyzed using SPSS version 13.0 (SPSS Inc., Chicago, IL, USA) and EpiInfo version 3.3.2 (Centers for Disease Control and Prevention, Atlanta, GA, USA).
At least 1 × 105 Th cells were acquired on a flow cytometer (FACS Aria/FACS LSRII BD BiosciencesSan Jose, CA CA, USA) and were analyzed using FlowJo software (Treestar, Inc. Ashland, OR, USA).
Ex vivo18F-FDG-labeled sections were exposed to phosphor films and read using the Cyclone Phosphor Imaging System (Packard Instruments, Meriden, CT, USA) and were analyzed using the OptiQuant software (Packard Instruments).
