The Epocrates Honors panel is an opt-in panel of 145 000 physicians in the USA verified by the American Medical Association's (AMA's) master file.
Values from each biological replicate were transferred into the statistical analysis program GraphPad (GraphPad Software, Inc., La Jolla, CA, USA), verified for normality, and an analysis of variance (ANOVA) run to assay differences between experimental treatments.
Data forms were created with Epi Info version 7 (CDC, Washington DC, USA), verified by ensuring that the study identities were matching the data in the questionnaires and prior to exporting to Statistical Package for Social Scientists (SPSS) version 16.0 (IBM, USA), where both descriptive and inferential analyses were performed.
SET-2, KG-1, F36-P, HEL92.1.7, THP-1, MV4-11, MOLM-13, ML-2, ME-1, SH-2, HL-60, MOLM-16, 32D, K562, KARPAS-1106P and RS4 11 cells were all cultivated according to the vendor's instructions, tested for mycoplasma contamination (Mycoplasma Plus PCR Primer Set, Stratagene; Agilene Technologies Inc., Santa Clara, CA, USA) and verified by STR profiling (John Hopkins University, Baltimore, MD, USA).
PCR products were purified with a gel extraction kit (Epoch Life Sciences, Sugar Land, TX, USA), cloned into pENTR™/D-TOPO® vector or pDONR™/Zeo vector (Invitrogen, Carlsbad, CA, USA), and verified by sequencing using M13 forward and reverse primers, and additional gene specific primers if necessary.
The observations obtained from thirteen states in USA was verified by the present model.
The RNA quality was analyzed with the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and verified to have a RNA integrity number ≥6.
The amplified cDNA was sub cloned into the EcoRI and HindIII restriction site of the pMALc2x vector (NEB, Beverly, MD, USA) and verified by automated nucleotide sequencing.
Finally the products were cloned into PCR2.1 vector (life technologies, Carlsbad, USA) and verified by sequencing.
Site-directed mutagenesis was performed using the QuikChangeII system (Stratagene, La Jolla, CA, USA) and verified by sequencing.
The SNP genotype callings were automatically performed using ABI GeneMapper 4.0 (Applied Biosystems, Foster City, CA, USA) and verified manually.
