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The ATR-FTIR spectra were collected with a Bruker Tensor 27 FTIR spectrometer (Billerica, MA, USA) using the standard Pike ATR cell.
FTIR measurement was recorded on a Bruker Vector 22 spectrophotometer (Madison, WI, USA) using the standard KBr disk method in the range of 4000 to 500 cm−1.
The total RNA was extracted with Trizol (Invitrogen, Carlsbad, California, USA), using the standard method.
CA-mediated protein carbonylation was estimated with an OxyBlot™ Protein Oxidation Detection Kit (Millipore Corporation, USA) using the standard protocol according to the manufacturer's instructions.
Real time PCR analysis was performed on an ABI Prism 5700 apparatus (Applied Biosystems, Foster City, CA, USA) using the standard protocol.
DNA was isolated from the swabs by automated DNA extraction on an Abbott M2000 sp (Abbott, Illinois, USA) using the standard Chlamydia trachomatis/Neisseria gonorrhoeae DNA extraction protocol as advised by the manufacturer.
For each sample, RNA was converted into cDNA (qScript™ cDNA SuperMix, Quanta Biosciences, USA), and mRNA quantification was determined (PerfeCTa® qPCR FastMix®, Quanta Biosciences, USA) using the two standard curves method with RPLP0 as a reference gene [ 16].> -wrap-foot> asthmant asthma or past documented clinical history of asthma.
Amino acid derivatives were separated by UPLC (Waters Corp., Milford, MA, USA) using the AccQ-Tag™ Ultra standard hydrolysate conditions.
For data acquisition an electronic case report form was created in OpenClinica (OpenClinica, LLC, Waltham, MA, USA) using the clinical data interchange standards consortium operational data model (CDISC, Austin, TX, USA).
All fragment analysis was conducted at Cornell University's Life Sciences Core Laboratory Center on an 3730xl DNA analyzer (Applied Biosystems, Carlsbad, California, USA) using the GeneScan 500 LIZ Size Standard (Applied Biosystems).
Immunohistochemical staining for cyclin D1 and Ki-67 was performed using a BenchMark automated stainer (Ventana, Tucson, AZ, USA) using the avidin biotin peroxidase technique following standard protocols.
