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Cells were cultured on growth factor-reduced Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) using the overlay method [ 17].
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Phage presence in the enrichments was confirmed via plaque formation using the overlay method described above.
The resulting network was expanded using the 'Overlay' function with IPA's 'Ingenuity Tox List'.
Known 'Functions' and 'Canonical Pathways' from IPA were mapped to the signature using the 'Overlay' function.
λ phosphatase markedly reduced interaction of 14-3-3 14-3-3 14-3-3sessed using the overlay assay.
The length of mitochondrial bundles was measured using the 'Overlay' and 'Measure' options of the Zeiss LSM Image Browser.
Open the image with SigmaScan and use the overlay draw mode of SigmaScan (Mode > Overlay draw mode) to mark ring boundaries with a dot.
Users are also invited to explore content on the app by using the "tag overlay".
Each dilution was titrated using the agar overlay method.
Bacteriophages were titrated using the agar overlay method, as described above.
Spheroids were initiated using the liquid overlay technique.
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