Exact(20)
The supernatant was then incubated in a 96 well (Nunc MaxiSorp, VWR, CA, USA) plate overnight at 4 °C.
The plates were incubated under microaerophilic conditions for 3 days at 37 °C on Mueller Hinton II Agar (Becton Dickinson, Franklin Lakes, NJ, USA) plate supplemented with 7%% horse blood without antibiotics.
The supernatant was then incubated in a 96 well (Nunc MaxiSorp, VWR, CA, USA) plate overnight at 4°C.
Aluminum adhesive plate seals were applied with an Abgene Seal-IT 100 (Rochester, NY, USA) plate sealer and plates were stored at −20°C in a Matrical MatriMinistore™ (Spokane, WA, USA) automated compound storage and retrieval system.
The OD at 600 nm of each well was measured using a Beckman (Palo Alto, CA, USA) plate reader.
Optical densities were read in triplicate at 560 nm using a Bio-Rad Laboratories (HerCAles, CA, USA) plate reader.
Similar(40)
Mueller-Hinton agar (Bacton Dickinson and Company, Cockeysville USA) plates were prepared according to the manufacturer guidelines.
Mueller-Hinton agar (Bacton Dickinson and Company, Cockeysville, MD, USA) plates were prepared according to the manufacturer guidelines.
Aliquots of 0.1 mL of the serial dilutions were seeded onto tryptone soya agar (TSA; Difco, Detroit, MI, USA) plates in duplicate by spread plate technique.
Three different fixed angle long volar plates were used: DVR (Hand Innovation LLC, Miami, FL, USA) plates in 11 cases, Acu Lock (Acumed®) in 4, and LCP Synthes plates (Synthes Ltd). in the remaining 6 cases.
Mice were sacrificed at various time points and organs were harvested, homogenised in 0.2 µm filtered PBS containing 0.05% Tween 80 and plated onto 7H11 Middlebrook (Difco™ USA) plates containing 10% oleic acid, albumin, dextrose and catalase (OADC) (Difco™ USA).
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