The solution was then poured drop by drop into deionized water (20 mL) under ultrasonic agitation using a sonifier (Branson Ultrasonics Co., Danbury, CT, USA) at a power level of 3 for 10 min. The organic solvents namely chloroform and methanol were then completely removed by vacuum distillation using a rotary evaporator.
All the hypotheses testing were implemented in STATISTICA 8.0 (StatSoft, Inc., Tulsa, OK, USA) at a significance level of 0.05.
The analyses were conducted with SPSS software version 21 (IBM, New York City, NY, USA) at a significance level of 0.05.
Measurements were compared using a paired Student's t-Test (Statistica Release 6, StatSoft Inc. Tulsa, USA) at a significance level of 0.05.
Data were analysed with Statistica software for Windows (StatSoft 2008, STATISTICA data analysis software system, version 8.0, http://www.statsoft.com, USA) at a significance level 0.05.
Regression analysis was performed using Statgraphics Plus v. 4, (StatPoint Technologies, Inc., Warrenton, VA, USA) at a 95%% level of significance on the means of six samples per species.
All quantitative data were analyzed with one-way ANOVA (SPSS, v.16.0, IBM, NY, USA) at a significance level of 0.05 (α = 0.05).
Statistical analyses were performed using the SPSS statistical software package, version 16.0 (SPSS Inc., Chicago, IL, USA) at a significance level of P<0.05.
Spearman bivariate correlations among the pairs of all 20 initial parameters [see Additional Information Table S1] were performed with SPSS Statistics (version 17.0, IBM® SPSS® Statistics, New York, NY, USA) at a 95%% level of significance and correlation coefficients were recorded [see Additional Information Table S2].
(SPSS software version 21, IBM, New York City, NY, USA) at an α level of 0.05.
