PCR products were sequenced on the 454 GS FLX Titanium platform (454 Life Sciences, Branford, CT, USA) to a depth of at least 2,600 reads per subject.
Injury was induced by moderate CCI using the eCCI-6.3 device (Custom Design and Fabrication, Richmond, VA, USA) at a velocity of 6 m/s, depth of 0.5 mm and 150 ms impact duration.
A data logger (HOBO U30, Onset, Massachusetts, USA) connected to a temperature probe (S-TMB-M006, Onset) at a depth of 10 cm and a soil water content probe (S-SMx-M005, Onset) covering a depth of 0 20 cm were used at each stand.
A fluorescent indicator of calcium (O6807; Oregon Green BAPTA-1 AM ester, Molecular Probes - Life Technologies, Grand Island, NY, USA) was bolus loaded by pressure injection into the hippocampus, at a depth of ∼100 µm.
For model calibration at Lautaret, soil moisture data at a depth of 0.1 m (sensor type: Echo-probe, Decagon Devices Inc., USA) was used.
5 × 10 GBM95 cells (or human astrocytes – control) were delivered in 3 μL DMEM-F12 at a depth of 3 mm with a Hamilton (Hamilton, Reno, Nevada, USA) syringe over 30 minutes.
Injections of chondroitinase ABC (ChABC; protease free, 15 U/ml in 0.9% saline, Sigma) were made at 1.5mm lateral to bregma, at a depth of ∼2mm, with glass pipettes connected to a pressure injector (WPI, USA).
At a depth of 700 metres, things are almost unchanged.
It was recovered at a depth of 14,000 feet.
PDT at a depth of 7.5 miles.
