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Primary human foreskin fibroblasts (HFF; ATCC, US) were maintained for less than 8 passages in Fibroblast Growth Medium plus Supplement (10% serum; PromoCell, Germany).
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What's really important for us is maintaining a sense of where you're at".
However, when primary mixed-effects models were repeated limited to US sites only, the results were maintained, suggesting that the data are robust.
Both lines were maintained using standard conditions.
Buildings were maintained more regularly.
Civil liberties were maintained.
Mice were maintained under sterile conditions.
Age-matched rats were maintained as controls.
All incubation times were maintained constant.
Embryos were maintained as standard method.
All mice were maintained under SPF conditions.
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