Sentence examples for us containing from inspiring English sources

After fixing the invaded cells with 3.7% formaldehyde, the porous membranes were removed form the invasion chamber using a scalpel blade and mounted onto a glass microscope slide using Vectashield (Molecular Probes, Eugene, OR, US) containing the nuclear stain 4',6-diamidino-2-phenylindole.

Tweets containing the following attributes were not used for analysis: if located outside the United States, from a user with a time zone outside the US, containing less than five words, not in English, not containing ASCII characters, and those submitted through the "API".

Watch: Raw Coverage of the KKK's Confederate Flag Rally in South Carolina.

In brief, the microarray slides were overlaid with the IgG or the IgM diluted in casein (Pierce, Illinois, US) containing 1% (w/v) BSA (Sigma-Aldrich) and 10 mM CaCl2, to give a final concentration of 0.1 and 0.5 µg/ml, respectively.

The targeting construct was sub-cloned into a backbone vector pSP72 (Promega, US) containing an ampicillin selection cassette for re-transformation of the construct prior to electroporation.

Cells were cultured in Dulbecco's modified Eagle's medium (Life Technologies, Bedford, MA, US) containing 10%% heat-inactivated fetal bovine serum, 100 units/mL penicillin, and 100 units/mL streptomycin in a humid chamber at 37 °C with 5%% CO2.

The commercial enzyme cocktail GC 220 (Genencor-Danisco, Rochester, NY, US) contained cellulase (filter paper units), cellobiase and xylanase activities of 116, 215 and 677 U/ml, respectively [ 22], with a specific gravity of 1.2 g/ml.

Therefore, the cereals used by us contain a complex natural mixture of polyphenols of different size, polarity and solubility.

It is important to point out that the cereal fractions used by us contain other compounds, both nutrients and non-nutrients, such as vitamins, minerals or fibre and, therefore, we cannot rule out that they can result in health benefits.

The Freud Museum, he reminds us, contains an extraordinary history.

The shelter, Gardiner tells us, contained furniture from all over the palace, and valuable Dutch paintings.