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Stool and urine specimens were assayed for S. haematobium, S. mansoni and geohelminths using standard procedures [ 39, 40].
Stool and urine specimens were assayed for S. haematobium, S. mansoni and geo-helminths using standard procedures [ 25, 26] and the intensities of S. haematobium calculated from at least 2 urine samples (maximum 3) collected on consecutive days, and those of S. mansoni calculated from mean intensities of at least 4 Kato-Katz slides (maximum 6), 2 from each of the 2 3 stool samples collected.
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All specimens were assayed in triplicate.
The detection rates in urine specimens were much lower than those in fecal specimens in all 3 assays.
The aMTS6 levels in nocturnal urine specimens were determined in duplicate using a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit from DRG Instruments GmbH (Marburg, Germany).
All urine specimens were diluted to achieve concentration for optimal density before performing the ELISA assay to fit the concentrations of respective NGAL protein in the linear range of the standard curve.
The inter-assay coefficients of variation for iAs, MMA, and DMA for the in-house reference urine specimens were 6.0%, 6.5%, and 5.9%.
All urine specimens were diluted to achieve concentration for optimal density according to the ELISA kit instruction before performing an ELISA assay to fit the concentrations of respective NGAL protein in the linear range of the standard curve.
Stool and urine specimens were collected on three consecutive days.
Urine specimens were treated similarly.
Blood and urine specimens were also collected.
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