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To compare the validity of the urinalysis on clean-voided bag versus catheter urine specimens using the catheter culture as the "gold" standard.
DNA was isolated from pooled urine specimens using the MagNA Pure LC Bacterial DNA isolation Kit III (Roche Molecular Systems, Alameda, USA) and amplified by polymerase chain reaction (PCR) (Cobas Amplicor, Roche Molecular Diagnostics, Branchburg, USA) [ 31].
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Basal glycaemia, glycosylated haemoglobin (HbA1c), haemoglobin, total cholesterol, LDL cholesterol, HDL cholesterol, non-HDL cholesterol, triglycerides, serum creatinine after overnight fasting for at least 12 hr, and the UACR in a urine sample collected in the first morning urine specimen, using the most recent value of the last 12 months.
Therefore: (1) we will monitor the location of urine specimens using a web-based database.
Drug tests for cases were performed on blood and/or urine specimens using radioimmunoassay techniques and liquid/gas chromatography coupled with mass spectometry (Kaplan et al. 2006; Li et al. 2011).
Initially, Gonorrhea and Chlamydia were detected using a rapid test kit (BioStar® OIA® GC and CHLAMYDIA) and positive samples were confirmed on urine specimens using TMA (Genprobe, San Diego, CA).
Weinreb draws attention to his recent report (Weinreb et al, 2006) in which decoy cells were identified in urine cytology specimens (using the Papinicolaou method) of apparently immunocompetent people who subsequently developed bladder cancer.
The serum and urine specimens used in this study were collected as part of a pneumonia etiology study that included HIV-infected patients in Thailand, described elsewhere [ 18].
The urinalysis was done on the uncentrifuged first aliquot (2 mls) of urine specimen using Combi 9 test strips by one of the authors.
To address this issue, we have analyzed RNA derived from urine specimen using Life technologies' TLDA-A and Illumina's NGS platforms.
Quantitative BKV results from a previously validated, laboratory-developed real-time PCR assay based on commercially available MGB Alert® reagents were compared to results obtained from the same urine and plasma specimens using a commercially designed real-time PCR assay by IntelligentMDx.
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