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Serum and urine samples were thawed and vortex mixed.
Urine samples were thawed to room temperature and filtered over 0.45 µm syringe filters.
Initially, the frozen urine samples were thawed at room temperature and then were centrifuged for 15 min at 4000 rpm.
For NMR analysis, urine samples were thawed at room temperature, and 500 µl of urine was mixed with 50 µl of phosphate buffer (1.5 M K2HPO4/1.5 M NaH2PO4, pH = 7.4).
Urine samples were thawed at room temperature.
The urine samples were thawed and shaken.
Similar(35)
Urine and saliva samples were thawed before the analysis, thoroughly mixed, and transferred into 2.0 mL polypropylene tubes.
Frozen samples were thawed at 4 °C, and 50 µL of each sample was diluted with 80% methanol (high-performance liquid chromatography grade, Millipore, Bedford, MA, USA) for plasma and 10% methanol for urine samples.
Snow samples were thawed overnight at 21 °C.
For analysis, samples were thawed.
The plasma samples were thawed at room temperature.
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