Sentence examples for urine samples were mixed from inspiring English sources

In brief, urine samples were mixed with the indicated array-specific binding buffer and loaded randomly in duplicate onto the pre-equilibrated ProteinChip surface, in order to minimize any error source.

Urine samples were mixed in a ratio of 3 1 with a solution of 20% Na3PO4.12H2O.

Before the measurements, thawed plasma and urine samples were mixed by inversion, centrifuged with cooling (1500 rpm for 10 min, 4°C, or 2000 rpm for 5 min, 4°C, resp).

In this study, 0.6 mL of CSF, 0.5 mL of plasma, and 0.05 mL of urine samples were mixed with 100 pg, 250 pg, and 330 pg of the internal standards, respectively, for each sample preparation.

Urine samples were mixed (1 12) with an α-cyano-4-hydroxycinnamic acid solution in 50%acetonitrile/0.1%% trifluoroacetic acid (TFA).

Original and manipulated urine samples were mixed with phosphate buffer (0.8 M Na2HPO4/NaH2PO4 pH 7.4 with 9% D2O and 50 μM DSS), at a ratio of one part buffer with two parts urine.

Urine samples were first mixed in a 2 1 ratio with sample buffer containing Brom Phenol Blue and protein denaturing 6% SDS (pH 6.8), and preincubated for 30 min. Samples were then loaded (15 μl) into the wells of a 10% gelatin gel G-26255, Sigma Chemicals Co., St Louis, MO, USA) and electrophoresed at 4°C to avoid spontaneous activation.

Urine samples were prepared by mixing urine (80 100 µL) with phosphate buffer (600 620 µL, pH 7.4, 0.2 M) containing 10% D2O as a field frequency lock and 0.05% sodium 3-trimethylsilylpropionate-2,2,3,3-d4 (TMSP, internal standard) as a chemical shift reference.

For this step, and, in a similar manner to the hair samples, the serum and urine samples were also neutralised by mixing with 1 mL of 0.2 M phosphate buffer (pH 7.0).

Urine samples were pooled and 10 μg of total proteins for each group were mixed with the Laemmli sample buffer (62.5 mM Tris HCl, pH 6.8, 25% glycerol, 2% SDS, 0.01% bromophenol blue) plus 0.5% dithiotreitol (DTT).

For NMR analysis, urine samples were thawed at room temperature, and 500 µl of urine was mixed with 50 µl of phosphate buffer (1.5 M K2HPO4/1.5 M NaH2PO4, pH = 7.4).