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Urine samples were maintained at −80 °C and were later thawed, centrifuged, and assayed directly by enzyme-linked immunoassay (ELISA).
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Samples were maintained under natural light conditions.
These urine samples were refrigerated or maintained on ice before processing in the lab and then stored at –20°C until pesticide metabolite analysis was performed in the National Center for Environmental Health at the CDC in Atlanta, Georgia, using a liquid chromatography/tandem mass spectrometry method (Olsson et al. 2004).
All patients and controls were instructed to give clean-catch urine samples, which were maintained at 4°C for a maximum of 3 hours.
All individuals were instructed to give clean-catch urine samples, which were maintained at 4°C for a maximum of 3 h.
Urine samples were collected daily, refrigerated or maintained on ice before processing in the lab, and then stored at –20°C until pesticide metabolite analysis was performed (Olsson et al. 2004) at the National Center for Environmental Health in the Centers for Disease Control and Prevention in Atlanta, Georgia.
Fasting urine samples were collected in the morning after an initial void and a wait of two hours, while participants maintained a fast from the evening before.
On indicated days, blood and urine samples were collected.
The urine samples were analyzed to determine arsenic metabolite concentrations.
Urine samples were analyzed for nitrogen.
However Rodchenkov said that as many as 100 dirty urine samples were expunged during the Games.
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